Structural Dna Nanotechnology

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ISBN: 0521764483
File Size: 14,89 MB
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Minimalist Strategies For Structural Dna Nanotechnology

Author: Kai Lin Lau
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ISBN:
File Size: 45,61 MB
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"With remarkable molecular recognition properties, DNA is an excellent material for bottom-up self-assembly in nanotechnology. A prominent example is the remarkable control and complexity provided by DNA origami. However, this complexity requires large sequence space in design. This translates to high cost of starting materials and error-prone assembly; hundreds of oligonucleotides are necessary, with reduced sequence uniqueness within oligonucleotides. The focus of this thesis - developing minimalist approaches to structural DNA nanotechnology - therefore seeks to address these issues by creating structures of functional simplicity. Structural complexity and uniqueness would be designed only where necessary, as dictated by function or application. Another correlated aim is additional reduction of complexity in assembly processes. This forms the conceptual framework for research presented herein.Chapter 2 demonstrates utility of a long templating strand in assembling DNA nanotubes with minimalist 3D triangular rungs. The strand is prepared though enzymatic means, and allows periodic rung assembly with good size-control. These structures can organize gold nanoparticles (AuNPs), with potential application in nanophotonics and nanoelectronics. Furthermore, direct conjugation of AuNPs to prefabricated 3D rungs is shown to greatly streamline the assembly process. In Chapter 3, molecular biology tools enable sequential growth preparation of extended templating strands featuring exact sequence and size-control. Through these, well-defined arrays of double-crossover (DX) and triple-crossover (TX) tiles are assembled, featuring periodic patterning, or specifically designed aperiodicity. These 1D structures truly rival the functionality of DNA origami, whilst using significantly less sequence space. Subsequently, strategies in creating more complex 2D assemblies with sequentially grown templating strands are explored in Chapter 4. Lateral width is engineered into minimalist tiles; 1D DX tile structures are hierarchically assembled into higher-order arrays; and tile nanorings are formed from circular ligation of template strands. Chapter 5 then describes investigation of minimal approaches in the DNA-directed organization of AuNPs. Encapsulation of AuNPs into a minimal wireframe cube is attempted, and AuNP dimerization is shown through an extremely simplified pre-fabricated double-stranded DNA template, as well as through simple controlled poly-functionalization of larger AuNPs. These minimal assemblies and their preparation are expected to have numerous potential application given the multitude of interesting properties and applications relevant to AuNPs.Throughout this work, key concepts can be discerned - templation, prefabrication, multi-purpose components, and molecular biology tools. Though these concepts are by no means requirements for minimalism, we believe their application can help guide future development of minimalist strategies for structural DNA nanotechnology." --

Structural Dna Nanotechnology With Novel Components

Author: Liang Ding
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ISBN:
File Size: 32,37 MB
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Structural Dna Nanotechnology

Author: Lisa B. Israel-Dreilinger
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ISBN:
File Size: 52,24 MB
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Dna Nanoscience

Author: Kenneth Douglas
Publisher: CRC Press
ISBN: 1315351404
File Size: 71,54 MB
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DNA Nanoscience: From Prebiotic Origins to Emerging Nanotechnology melds two tales of DNA. One is a look at the first 35 years of DNA nanotechnology to better appreciate what lies ahead in this emerging field. The other story looks back 4 billion years to the possible origins of DNA which are shrouded in mystery. The book is divided into three parts comprised of 15 chapters and two Brief Interludes. Part I includes subjects underpinning the book such as a primer on DNA, the broader discipline of nanoscience, and experimental tools used by the principals in the narrative. Part II examines the field of structural DNA nanotechnology, founded by biochemist/crystallographer Nadrian Seeman, that uses DNA as a construction material for nanoscale structures and devices, rather than as a genetic material. Part III looks at the work of physicists Noel Clark and Tommaso Bellini who found that short DNA (nanoDNA) forms liquid crystals that act as a structural gatekeeper, orchestrating a series of self-assembly processes using nanoDNA. This led to an explanation of the polymeric structure of DNA and of how life may have emerged from the prebiotic clutter.

Uniquimer 3d A Software System For Structural Dna Nanotechnology Design Analysis And Evaluation

Author: Jinhao Zhu
Publisher:
ISBN:
File Size: 55,67 MB
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Dna In Supramolecular Chemistry And Nanotechnology

Author: Eugen Stulz
Publisher: John Wiley & Sons
ISBN: 1118696948
File Size: 50,93 MB
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This book covers the emerging topic of DNA nanotechnology and DNA supramolecular chemistry in its broader sense. By taking DNA out of its biological role, this biomolecule has become a very versatile building block in materials chemistry, supramolecular chemistry and bio-nanotechnology. Many novel structures have been realized in the past decade, which are now being used to create molecular machines, drug delivery systems, diagnosis platforms or potential electronic devices. The book combines many aspects of DNA nanotechnology, including formation of functional structures based on covalent and non-covalent systems, DNA origami, DNA based switches, DNA machines, and alternative structures and templates. This broad coverage is very appealing since it combines both the synthesis of modified DNA as well as designer concepts to successfully plan and make DNA nanostructures. Contributing authors have provided first a general introduction for the non-specialist reader, followed by a more in-depth analysis and presentation of their topic. In this way the book is attractive and useful for both the non-specialist who would like to have an overview of the topic, as well as the specialist reader who requires more information and inspiration to foster their own research.

Gideon

Author: Jeffrey J. Birac
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ISBN:
File Size: 80,91 MB
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Nucleic Acids From A To Z

Author: Sabine Müller
Publisher: John Wiley & Sons
ISBN: 9783527312115
File Size: 42,59 MB
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Concise but complete, this mini-encyclopedia contains over 1,500 entries covering all important concepts, compounds, techniques and acronyms for quick and easy reference. Guiding readers through the ever-increasing jungle of nucleic acid science and technology, the book distills the key information out of the large body of primary literature and presents it in a single volume. A first-stop resource for everyone, from students to established researchers, as both a desktop and library reference.

Rna Nanotechnology

Author: Bin Wang
Publisher: CRC Press
ISBN: 9814411655
File Size: 48,53 MB
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In the past few decades there has been incredible growth in "bionano"-related research, which has been accompanied by numerous publications in this field. Although various compilations address topics related to deoxyribonucleic acid (DNA) and protein, there are few books that focus on determining the structure of ribonucleic acid (RNA) and using RNA as building blocks to construct nanoarchitectures for biomedical and healthcare applications. RNA Nanotechnology is a comprehensive volume that details both the traditional approaches and the latest developments in the field of RNA-related technology. This book targets a wide audience: a broad introduction provides a solid academic background for students, researchers, and scientists who are unfamiliar with the subject, while the in-depth descriptions and discussions are useful for advanced professionals. The book opens with reviews on the basic aspects of RNA biology, computational approaches for predicting RNA structures, and traditional and emerging experimental approaches for probing RNA structures. This section is followed by explorations of the latest research and discoveries in RNA nanotechnology, including the design and construction of RNA-based nanostructures. The final segment of the book includes descriptions and discussions of the potential biological and therapeutic applications of small RNA molecules, such as small/short interfering RNAs (siRNAs), microRNAs (miRNAs), RNA aptamers, and ribozymes.